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sirna template design tool  (Thermo Fisher)


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    Thermo Fisher sirna template design tool
    Gene and protein expressions after the <t>siRNA</t> silencing of HSP27, cFLIP, <t>and</t> <t>CLU</t> genes in PC-3 cells. The efficiency of siRNA knockdown was verified by analyzing mRNA and protein levels by qRT-PCR and Western blotting, respectively. ( A ) After the siRNA treatment, the mRNA expression of HSP27, cFLIP, and CLU was significantly reduced. Data represent the mean ± SEM of three independent experiments, each conducted in triplicate. ( B ) siRNA-treated cells show a corresponding decrease in the protein expression of these genes.
    Sirna Template Design Tool, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sirna+template+design+tool/pmc10971947-45-22-26
    Average 90 stars, based on 1 article reviews
    sirna template design tool - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Triple Silencing of HSP27, cFLIP, and CLU Genes Promotes the Sensitivity of Doxazosin-Induced Apoptosis in PC-3 Prostate Cancer Cells"

    Article Title: Triple Silencing of HSP27, cFLIP, and CLU Genes Promotes the Sensitivity of Doxazosin-Induced Apoptosis in PC-3 Prostate Cancer Cells

    Journal: Medicines

    doi: 10.3390/medicines11030007

    Gene and protein expressions after the siRNA silencing of HSP27, cFLIP, and CLU genes in PC-3 cells. The efficiency of siRNA knockdown was verified by analyzing mRNA and protein levels by qRT-PCR and Western blotting, respectively. ( A ) After the siRNA treatment, the mRNA expression of HSP27, cFLIP, and CLU was significantly reduced. Data represent the mean ± SEM of three independent experiments, each conducted in triplicate. ( B ) siRNA-treated cells show a corresponding decrease in the protein expression of these genes.
    Figure Legend Snippet: Gene and protein expressions after the siRNA silencing of HSP27, cFLIP, and CLU genes in PC-3 cells. The efficiency of siRNA knockdown was verified by analyzing mRNA and protein levels by qRT-PCR and Western blotting, respectively. ( A ) After the siRNA treatment, the mRNA expression of HSP27, cFLIP, and CLU was significantly reduced. Data represent the mean ± SEM of three independent experiments, each conducted in triplicate. ( B ) siRNA-treated cells show a corresponding decrease in the protein expression of these genes.

    Techniques Used: Quantitative RT-PCR, Western Blot, Expressing

    Effect of doxazosin on the viability of PC-3 cells subjected to single or dual siRNA silencing of HSP27, cFLIP, and CLU genes. PC-3 cells were treated with 0, 10, or 25 µM doxazosin for 24 h, and then cell viability was measured by the MTT assay. ( A ) Cell viability was significantly decreased at 25 µM doxazosin in cells with CLU gene silencing, while the silencing of HSP27 and cFLIP genes did not show this effect. ( B ) Dual siRNA silencing (HSP27 and cFLIP, HSP27 and CLU, or cFLIP and CLU) resulted in a significant decrease in cell viability at both 10 and 25 µM doxazosin. Data represent the mean ± SEM of three independent experiments, each conducted in triplicate. Statistical significance ( p < 0.05) was determined by a one-way ANOVA and Tukey’s test followed by a Student’s t -test for 0 µM vs. 1, 10, and 25 µM comparisons.
    Figure Legend Snippet: Effect of doxazosin on the viability of PC-3 cells subjected to single or dual siRNA silencing of HSP27, cFLIP, and CLU genes. PC-3 cells were treated with 0, 10, or 25 µM doxazosin for 24 h, and then cell viability was measured by the MTT assay. ( A ) Cell viability was significantly decreased at 25 µM doxazosin in cells with CLU gene silencing, while the silencing of HSP27 and cFLIP genes did not show this effect. ( B ) Dual siRNA silencing (HSP27 and cFLIP, HSP27 and CLU, or cFLIP and CLU) resulted in a significant decrease in cell viability at both 10 and 25 µM doxazosin. Data represent the mean ± SEM of three independent experiments, each conducted in triplicate. Statistical significance ( p < 0.05) was determined by a one-way ANOVA and Tukey’s test followed by a Student’s t -test for 0 µM vs. 1, 10, and 25 µM comparisons.

    Techniques Used: MTT Assay

    Effect of doxazosin on the viability of PC-3 cells subjected to triple siRNA silencing of HSP27, cFLIP, and CLU genes. Cell viability was significantly decreased at 10 and 25 μM doxazosin. In addition, even at 1 μM doxazosin, cell viability was significantly reduced compared to cells subjected to dual siRNA targeting two genes. Data represent the mean ± SEM of three independent experiments, each conducted in triplicate. Statistical significance ( p < 0.05) was determined by a one-way ANOVA and Tukey’s test followed by a Student’s t -test for 0 µM vs. 1, 10, and 25 µM comparisons.
    Figure Legend Snippet: Effect of doxazosin on the viability of PC-3 cells subjected to triple siRNA silencing of HSP27, cFLIP, and CLU genes. Cell viability was significantly decreased at 10 and 25 μM doxazosin. In addition, even at 1 μM doxazosin, cell viability was significantly reduced compared to cells subjected to dual siRNA targeting two genes. Data represent the mean ± SEM of three independent experiments, each conducted in triplicate. Statistical significance ( p < 0.05) was determined by a one-way ANOVA and Tukey’s test followed by a Student’s t -test for 0 µM vs. 1, 10, and 25 µM comparisons.

    Techniques Used:

    Related Articles

    Sequencing:

    Article Title: Frizzled-7 dictates three-dimensional organization of colorectal cancer cell carcinoids.
    Article Snippet: .. RNAi targeting sites in the human FZD7 coding sequence (accession number NM_003507) were selected using the siRNA template design tool (Ambion, Austin, TX, USA) and verified by Blast search. .. Short hairpin (sh) RNA coding oligos were then designed and cloned into the BglII and HindIII sites of the pRETROSUPER vector (generous gift from Reuven Agami, Amsterdam, The Netherlands) as described (Brummelkamp et al., 2002; Eckhardt et al., 2005).

    Immunoprecipitation:

    Article Title: PPAR?-Regulated Tight Junction Development During Human Urothelial Cytodifferentiation
    Article Snippet: .. The immunoprecipitated protein was eluted from the column in a denaturing elution buffer (Catch and Release kit, Upstate) and resolved on SDS–PAGE by western blot analysis. siRNA siRNA templates were designed for three regions that were homologous for PPAR γ 1 ( {"type":"entrez-nucleotide","attrs":{"text":"NM_138712","term_id":"116284369","term_text":"NM_138712"}} NM_138712 ), PPAR γ 2 ( {"type":"entrez-nucleotide","attrs":{"text":"NM_015869","term_id":"116284371","term_text":"NM_015869"}} NM_015869 ), PPAR γ 3 ( {"type":"entrez-nucleotide","attrs":{"text":"NM_138711","term_id":"116284372","term_text":"NM_138711"}} NM_138711 ) and PPAR γ 4 ( {"type":"entrez-nucleotide","attrs":{"text":"NM_005037","term_id":"116284367","term_text":"NM_005037"}} NM_005037 ) using the Ambion siRNA template design tool ( www.ambion.com ). siRNA templates were for region 1: 5′-AAGACATTCCATTCA-CAAGAACCTGTCTC-3′ and 5′-AATTCTTGTGAATGGAAT-GTCCCTGTCTC-3′; region 2: 5′-AAATCACCATTCGTTA-TCTATCCTGTCTC-3′ and 5′-AAATAGATAACGAATGGT-GATCCTGTCTC-3′; region 3: 5′-AATCTGACACCTAA-GAAATTTCCTGTCTC-3′ and 5′-AAAAATTTCTTAGGTG-TCAGACCTGTCTC-3′. .. The templates were used to make PPARγ siRNA complexes using the Silencer TM siRNA construction kit (Ambion Ltd., Huntingdon, Cambridgeshire, UK).

    Western Blot:

    Article Title: PPAR?-Regulated Tight Junction Development During Human Urothelial Cytodifferentiation
    Article Snippet: .. The immunoprecipitated protein was eluted from the column in a denaturing elution buffer (Catch and Release kit, Upstate) and resolved on SDS–PAGE by western blot analysis. siRNA siRNA templates were designed for three regions that were homologous for PPAR γ 1 ( {"type":"entrez-nucleotide","attrs":{"text":"NM_138712","term_id":"116284369","term_text":"NM_138712"}} NM_138712 ), PPAR γ 2 ( {"type":"entrez-nucleotide","attrs":{"text":"NM_015869","term_id":"116284371","term_text":"NM_015869"}} NM_015869 ), PPAR γ 3 ( {"type":"entrez-nucleotide","attrs":{"text":"NM_138711","term_id":"116284372","term_text":"NM_138711"}} NM_138711 ) and PPAR γ 4 ( {"type":"entrez-nucleotide","attrs":{"text":"NM_005037","term_id":"116284367","term_text":"NM_005037"}} NM_005037 ) using the Ambion siRNA template design tool ( www.ambion.com ). siRNA templates were for region 1: 5′-AAGACATTCCATTCA-CAAGAACCTGTCTC-3′ and 5′-AATTCTTGTGAATGGAAT-GTCCCTGTCTC-3′; region 2: 5′-AAATCACCATTCGTTA-TCTATCCTGTCTC-3′ and 5′-AAATAGATAACGAATGGT-GATCCTGTCTC-3′; region 3: 5′-AATCTGACACCTAA-GAAATTTCCTGTCTC-3′ and 5′-AAAAATTTCTTAGGTG-TCAGACCTGTCTC-3′. .. The templates were used to make PPARγ siRNA complexes using the Silencer TM siRNA construction kit (Ambion Ltd., Huntingdon, Cambridgeshire, UK).



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    sirna template design tool  (Thermo Fisher)
    90
    Thermo Fisher sirna template design tool
    Gene and protein expressions after the <t>siRNA</t> silencing of HSP27, cFLIP, <t>and</t> <t>CLU</t> genes in PC-3 cells. The efficiency of siRNA knockdown was verified by analyzing mRNA and protein levels by qRT-PCR and Western blotting, respectively. ( A ) After the siRNA treatment, the mRNA expression of HSP27, cFLIP, and CLU was significantly reduced. Data represent the mean ± SEM of three independent experiments, each conducted in triplicate. ( B ) siRNA-treated cells show a corresponding decrease in the protein expression of these genes.
    Sirna Template Design Tool, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sirna+template+design+tool/pmc10971947-45-22-26
    Average 90 stars, based on 1 article reviews
    sirna template design tool - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Gene and protein expressions after the siRNA silencing of HSP27, cFLIP, and CLU genes in PC-3 cells. The efficiency of siRNA knockdown was verified by analyzing mRNA and protein levels by qRT-PCR and Western blotting, respectively. ( A ) After the siRNA treatment, the mRNA expression of HSP27, cFLIP, and CLU was significantly reduced. Data represent the mean ± SEM of three independent experiments, each conducted in triplicate. ( B ) siRNA-treated cells show a corresponding decrease in the protein expression of these genes.

    Journal: Medicines

    Article Title: Triple Silencing of HSP27, cFLIP, and CLU Genes Promotes the Sensitivity of Doxazosin-Induced Apoptosis in PC-3 Prostate Cancer Cells

    doi: 10.3390/medicines11030007

    Figure Lengend Snippet: Gene and protein expressions after the siRNA silencing of HSP27, cFLIP, and CLU genes in PC-3 cells. The efficiency of siRNA knockdown was verified by analyzing mRNA and protein levels by qRT-PCR and Western blotting, respectively. ( A ) After the siRNA treatment, the mRNA expression of HSP27, cFLIP, and CLU was significantly reduced. Data represent the mean ± SEM of three independent experiments, each conducted in triplicate. ( B ) siRNA-treated cells show a corresponding decrease in the protein expression of these genes.

    Article Snippet: The mRNA target sequences for HSP27 (Gene ID: 3315), cFLIP (Gene ID: 8837), and CLU (Gene ID: 1191) were designed using a siRNA template design tool (Ambion, Austin, TX, USA).

    Techniques: Quantitative RT-PCR, Western Blot, Expressing

    Effect of doxazosin on the viability of PC-3 cells subjected to single or dual siRNA silencing of HSP27, cFLIP, and CLU genes. PC-3 cells were treated with 0, 10, or 25 µM doxazosin for 24 h, and then cell viability was measured by the MTT assay. ( A ) Cell viability was significantly decreased at 25 µM doxazosin in cells with CLU gene silencing, while the silencing of HSP27 and cFLIP genes did not show this effect. ( B ) Dual siRNA silencing (HSP27 and cFLIP, HSP27 and CLU, or cFLIP and CLU) resulted in a significant decrease in cell viability at both 10 and 25 µM doxazosin. Data represent the mean ± SEM of three independent experiments, each conducted in triplicate. Statistical significance ( p < 0.05) was determined by a one-way ANOVA and Tukey’s test followed by a Student’s t -test for 0 µM vs. 1, 10, and 25 µM comparisons.

    Journal: Medicines

    Article Title: Triple Silencing of HSP27, cFLIP, and CLU Genes Promotes the Sensitivity of Doxazosin-Induced Apoptosis in PC-3 Prostate Cancer Cells

    doi: 10.3390/medicines11030007

    Figure Lengend Snippet: Effect of doxazosin on the viability of PC-3 cells subjected to single or dual siRNA silencing of HSP27, cFLIP, and CLU genes. PC-3 cells were treated with 0, 10, or 25 µM doxazosin for 24 h, and then cell viability was measured by the MTT assay. ( A ) Cell viability was significantly decreased at 25 µM doxazosin in cells with CLU gene silencing, while the silencing of HSP27 and cFLIP genes did not show this effect. ( B ) Dual siRNA silencing (HSP27 and cFLIP, HSP27 and CLU, or cFLIP and CLU) resulted in a significant decrease in cell viability at both 10 and 25 µM doxazosin. Data represent the mean ± SEM of three independent experiments, each conducted in triplicate. Statistical significance ( p < 0.05) was determined by a one-way ANOVA and Tukey’s test followed by a Student’s t -test for 0 µM vs. 1, 10, and 25 µM comparisons.

    Article Snippet: The mRNA target sequences for HSP27 (Gene ID: 3315), cFLIP (Gene ID: 8837), and CLU (Gene ID: 1191) were designed using a siRNA template design tool (Ambion, Austin, TX, USA).

    Techniques: MTT Assay

    Effect of doxazosin on the viability of PC-3 cells subjected to triple siRNA silencing of HSP27, cFLIP, and CLU genes. Cell viability was significantly decreased at 10 and 25 μM doxazosin. In addition, even at 1 μM doxazosin, cell viability was significantly reduced compared to cells subjected to dual siRNA targeting two genes. Data represent the mean ± SEM of three independent experiments, each conducted in triplicate. Statistical significance ( p < 0.05) was determined by a one-way ANOVA and Tukey’s test followed by a Student’s t -test for 0 µM vs. 1, 10, and 25 µM comparisons.

    Journal: Medicines

    Article Title: Triple Silencing of HSP27, cFLIP, and CLU Genes Promotes the Sensitivity of Doxazosin-Induced Apoptosis in PC-3 Prostate Cancer Cells

    doi: 10.3390/medicines11030007

    Figure Lengend Snippet: Effect of doxazosin on the viability of PC-3 cells subjected to triple siRNA silencing of HSP27, cFLIP, and CLU genes. Cell viability was significantly decreased at 10 and 25 μM doxazosin. In addition, even at 1 μM doxazosin, cell viability was significantly reduced compared to cells subjected to dual siRNA targeting two genes. Data represent the mean ± SEM of three independent experiments, each conducted in triplicate. Statistical significance ( p < 0.05) was determined by a one-way ANOVA and Tukey’s test followed by a Student’s t -test for 0 µM vs. 1, 10, and 25 µM comparisons.

    Article Snippet: The mRNA target sequences for HSP27 (Gene ID: 3315), cFLIP (Gene ID: 8837), and CLU (Gene ID: 1191) were designed using a siRNA template design tool (Ambion, Austin, TX, USA).

    Techniques: